Abstract
Recent genetic analyses have indicated that polymorphisms in the mouse APOBEC3 locus constitute the Rfv3 gene that influences the production of virus-neutralizing antibodies in mice infected with Friend leukemia retrovirus [1,2]. Mice of the resistant genotype preferentially express the exon 5-lacking transcript in higher levels, while susceptible mice express the full-length transcript in lower levels [2,3]. Mouse APOBEC3 expressed in the resistant strains restricts the replication of mouse retroviruses in vitro through a mechanism independent of its deaminase activity [2]. However, the mechanisms through which mouse APOBEC3 affects the production of virus-neutralizing antibodies remain unclear. To address this question, we analyzed retroviral replication and the production of virus-neutralizing antibodies in mice of different APOBEC3 genotypes and those lacking its expression. Strain A mice with the susceptible APOBEC3 genotype nevertheless produced high levels of virus-neutralizing antibodies when they possessed the H-2b haplotype, and class-switching to IgG was observed in the presence of virus-specific T helper cells. Further, APOBEC3-deficient mice produced virus-neutralizing antibodies when their T helper cells had been primed with the viral antigen. Friend virus-induced derangements in the hematopoiesis and resultant splenomegaly are not directly responsible for the delayed antibody responses, because higher levels of viremia and lower antibody responses were observed upon infection with nonpathogenic Friend murine leukemia helper virus in the absence of APOBEC3. In mice of the resistance-associated APOBEC3 genotype lower levels of viremia were observed even before the detection of virus-neutalizing antibodies in the blood, indicating that the polymorphisms in the APOBEC3 locus affect the production of neutralizing antibodies as a result of restricted retroviral replication.
Highlights
Mouse APOBEC3 affects the production of virus-neutralizing antibodies by restricting early retroviral replication, not by altering the B-cell repertoire
Recent genetic analyses have indicated that polymorphisms in the mouse APOBEC3 locus constitute the Rfv3 gene that influences the production of virus-neutralizing antibodies in mice infected with Friend leukemia retrovirus [1,2]
In mice of the resistance-associated APOBEC3 genotype lower levels of viremia were observed even before the detection of virus-neutalizing antibodies in the blood, indicating that the polymorphisms in the APOBEC3 locus affect the production of neutralizing antibodies as a result of restricted retroviral replication
Summary
Mouse APOBEC3 affects the production of virus-neutralizing antibodies by restricting early retroviral replication, not by altering the B-cell repertoire. Masaaki Miyazawa*, Sachiyo Tsuji-Kawahara, Tomomi Chikaishi, Maiko Kato and Shiki Takamura Address: Department of Immunology, Kinki University School of Medicine, Osaka-Sayama, Osaka, Japan 589-8511 * Corresponding author from Frontiers of Retrovirology: Complex retroviruses, retroelements and their hosts Montpellier, France.
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