Abstract
The TRP‐channel VRL‐1 is a heat‐sensitive, nonselective cation channel expressed by primary sensory neurons and non‐neuronal tissues. VRL‐1 is activated above ∼52°C but not by capsaicin or protons. It has been shown that F‐11 cells, hybridoma of mouse neuroblastoma and rat dorsal root ganglion cells, endogenously express VRL‐1 (1). We here present data on the characterisation of VRL‐1 in F‐11 cells at the nucleic acid and protein level. Anti‐VRL‐1 immunoreactivity showed up at ∼80 kDa in Western blots with F‐11 cell homogenate. By analysing overlapping PCR‐fragments with specific rat VRL‐1 primers we found that F‐11 cells express both rat and mouse VRL‐1. The F‐11 cell line provides a powerful experimental system to study VRL‐1 biochemistry, but one has to be aware that the mouse and the rat protein are expressed.
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