Abstract

The release kinetics of a single DNA molecule hooked around an obstacle in the presence of an electric field is investigated. A kinetic model which includes both electrical and stretching forces is introduced and used both in computer simulation and in the development of a simple analytic argument. These predict two regimes which depend on \ensuremath{\Upsilon}=N/\ensuremath{\beta}, where N is the number of chain segments and 1/\ensuremath{\beta} is the dimensionless field strength. For short chains, \ensuremath{\Upsilon}1, the characteristic unhooking time scales as ${\mathit{N}}^{2}$, whereas for long chains, \ensuremath{\Upsilon}\ensuremath{\gtrsim}1, it scales as N. Without introducing post friction, the model is able to reproduce recent experimental observations.

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