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Abstract
The chance of obtaining a fertilization and establishing a pregnancy increases with the number of motile spermatozoa that can reach and interact with the oocyte after the time of insemination. In an attempt to increase the recovery of freeze-thawed and motile spermatozoa, the swim-up medium was supplemented with prostasomes and some other effectors. Swim-up media supplemented with prostasomes were superior in comparison to the other effectors investigated in the recovery of motile spermatozoa for insemination. These results suggest that prostasome inclusion in swim-up medium might be of benefit in improving results in assisted reproductive technologies using freeze-thawed spermatozoa.
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