Abstract
The objective of this study was to test the use of a commercial extender (Triladyl) as a diluent in caprine semen refrigerated at 15℃, using caffeine (CF), heparin (HP), synthetic oviductal fluid (SOF) andtriladyl (TRY) as capacitating chemical agents at different times. Twenty ejaculates of caprine semen were collected using an artificial vagina. The ejaculates were diluted and refrigerated by three days. Evaluated the progressive motility (PM) and the functional state of the sperm plasma membrane trough fluorescent CTC staining, counting 200 spermatic cells (non-capacitated spermatozoa NCS, capacitated CS and reacting spermatozoa REA) of caprine in two capacitating agents: CAF and HEP; one culture medium:SOF and a commercial extender: TRY at 60, 120, 180 and 240 min of incubation, during 24, 48 and 72 hs. PM was high under TRY, and CS was high under the HEP treatment. TRY could be an alternative to capacitate caprine spermatozoa, keeping PM for a longer time than HEP or CAF.
Highlights
Artificial insemination (AI) is probably the reproductive technology most widely used, because it is simple and the highest benefit: Cost ratio when tested bucks are used for reproduction [1]
The objective of this study was to test the use of a commercial extender (Triladyl) as a diluent in caprine semen refrigerated at 15 ̊C, using caffeine (CF), heparin (HP), synthetic oviductal fluid (SOF) andtriladyl (TRY) as capacitating chemical agents at different times
A greater progressive motility (PM) and non-capacitated spermatozoa (NCS) spermatozoa was observed for TRY and SOF treatments (P < 0.01), respectively (Table 1)
Summary
Artificial insemination (AI) is probably the reproductive technology most widely used, because it is simple and the highest benefit: Cost ratio when tested bucks are used for reproduction [1]. Semen from tested bucks could be used fresh, freeze or frozen. Fresh semen must be used inmediately after its collection, because motility and viability of the spermatozoa is reduced in a short time. Frozen semen could be maintained longer than 48 hs [2], providing a greater flexibility of their use in AI programs. Frozen semen could be carry from the animal breeding center or a given ranch to the desire one [3]. Preservation of ovine semen kept at 15 ̊C [4] caprinesemen at 5 ̊C - 21 ̊C [5] and canine semen [6] has been notified. It has been found that the production of reactive oxygen species (ROS) occurs mainly during the cooling period at 5 ̊C [7]
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