Abstract
Manual measurement is unreliable as its highly subjective, leading to different estimates of motility between different laboratories. Further more, morphological determination is then made at different time on fixed, killed and stained sample. Several advanced sperm selection methods were currently used prior to ART, these are inadequate and fail to ensure spermatozoa with optimum quality. To assist in these microscopic measurements, several manufactures have developed and introduced into the market Computer Aided Sperm Analysis (CASA). These CASA machines can improve the accuracy of the microscopic measures, although major problem with them are: difficulty in tracking sperm trajectories, separate software is required to analyze sperm morphology and in addition high purchase costs, CASA machines are rarely used in routine laboratories. Clinician or researcher believe that the motile sperms are morphologically normal, whereas in reality this is not always the case. In this paper we have developed image analysis system using open source software IMAGEJ, which perform the test simultaneously to extract the sperm cells from the input video and perform the motility analysis with morphology on each sperm. Motility with morphology analysis using one-test procedure helps embryologist to improve fertility rate in assisted reproductive techniques.
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