Abstract
A map of transcription start sites across the Drosophila genome, providing insights into initiation patterns and spatiotemporal conditions.
Highlights
Transcription initiation is a key component in the regulation of gene expression. mRNA 5' full-length sequencing techniques have enhanced our understanding of mammalian transcription start sites (TSSs), revealing different initiation patterns on a genomic scale
Our analysis demonstrates that sequence elements in core promoters are directly associated with initiation patterns and the spatiotemporal conditions under which they are utilized
The Eukaryotic Promoter Database (EPD) incorporates highly confident TSSs identified from the curation of expressed sequence tag (EST) and is of a similar magnitude to our previous study [38]
Summary
Transcription initiation is a key component in the regulation of gene expression. mRNA 5' full-length sequencing techniques have enhanced our understanding of mammalian transcription start sites (TSSs), revealing different initiation patterns on a genomic scale. Transcription is a crucial part of gene expression that involves complex interactions of cis-regulatory sequence elements and trans-factors It is mediated in large part through the binding of transcription factors (TFs) to DNA sequence motifs. Recent high throughput sequencing efforts based on 5' capping protocols have generated capped transcripts for human and mouse on a high throughput scale under numerous conditions [5,6,7] These '5'-capped' or 'cap-trapped' transcripts have helped to identify genomic TSS locations for thousands of genes, in particular for human, mouse and yeast [8,9,10]. The large scale of available data allows for detailed analyses; for instance, one study explored the importance of precise spacing between the TATA box and the TSS [13]
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