Abstract
BackgroundSerial blood passage of Plasmodium increases virulence, whilst mosquito transmission inherently regulates parasite virulence within the mammalian host. It is, therefore, imperative that all aspects of experimental malaria research are studied in the context of the complete Plasmodium life cycle.MethodsPlasmodium chabaudi chabaudi displays many characteristics associated with human Plasmodium infection of natural mosquito vectors and the mammalian host, and thus provides a unique opportunity to study the pathogenesis of malaria in a single infection setting. An optimized protocol that permits efficient and reproducible vector transmission of P. c. chabaudi via Anopheles stephensi was developed.Results and conclusionsThis protocol was utilized for mosquito transmission of genetically distinct P. c. chabaudi isolates, highlighting differential parasite virulence within the mosquito vector and the spectrum of host susceptibility to infection initiated via the natural route, mosquito bite. An apposite experimental system in which to delineate the pathogenesis of malaria is described in detail.
Highlights
Serial blood passage of Plasmodium increases virulence, whilst mosquito transmission inherently regulates parasite virulence within the mammalian host
All available data from mosquito transmission of P. c. chabaudi over a one-year period (18 independent experiments) has been collated, and the results described derive from that pooled dataset, unless stated otherwise
It was noted in this study that the magnitude of peak parasitaemia is not influenced by the number of injected sporozoites, or the number of mosquito bites, that initiate infection
Summary
Serial blood passage of Plasmodium increases virulence, whilst mosquito transmission inherently regulates parasite virulence within the mammalian host. It is, imperative that all aspects of experimental malaria research are studied in the context of the complete Plasmodium life cycle. Mosquito transmission of Plasmodium directly regulates parasite virulence, and is an imperative in experimental malaria research. Chabaudi to distinct strains of laboratory mice This protocol is presented, and details the requirements of transmission and the expected outcomes of infection. Chabaudi permits analysis of parasite virulence, and vector and host responses to infection, in the context of the complete Plasmodium life cycle. An apposite experimental system in which to study many of the hallmark features of human disease is described
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