Mosquito larvicidal activity of silver nanoparticles synthesised using actinobacterium, Streptomyces sp. M25 against Anopheles subpictus, Culex quinquefasciatus and Aedes aegypti.

Abstract

The present work reports the larvicidal potential of microbially synthesised silver nanoparticles (AgNPs) by using an actinobacterium, Streptomyces sp. M25 isolated from Western Ghats, Tamil Nadu, India. The biomass of Streptomyces sp. was exposed to 1mM silver nitrate (AgNO3) solution. The synthesis of AgNPs was confirmed by visual inspection followed by instrumental analysis such as UV-vis spectroscopy, fourier transform infrared spectroscopy, X-ray diffraction (XRD), energy dispersive X-ray spectroscopy, scanning electron microscopy, atomic force microscopy and transmission electron microscopy (TEM) with selected area electron diffraction. Based on the TEM and XRD analysis, the average size of the AgNPs was determined to be 10-35nm. The biosynthesised AgNPs exhibited significant larvicidal activity against malarial vector, Anopheles subpictus (LC50 51.34mg/L and χ (2) value of 8.228), filarial vector, Culex quinquefasciatus (LC50 48.98mg/L and χ (2) value of 14.307) and dengue vector, Aedes aegypti (LC50 60.23mg/L and χ (2) value of 4.042), respectively. Similarly, AgNO3 had larvicidal activity against malarial vector, A. subpictus (LC50 42.544mg/L and χ (2) value of 2.561), filarial vector, C. quinquefasciatus (LC50 44.922mg/L and χ (2) value of 1.693) and dengue vector, A. aegypti (LC50 39.664mg/L and χ (2) value of 5.724), respectively. The current study is a rapid, cost effective, eco-friendly and single step approach. The Streptomyces sp. M25 is a newly added source for the synthesis of AgNPs with improved larvicidal activity.