Abstract
BackgroundMorus alba, a medicinal plant in Asia, has been used traditionally to treat diabetes mellitus and hypoglycemia. However, the effects of M. alba extract (MAE) on atopic dermatitis have not been verified scientifically. We investigated the effects of MAE on atopic dermatitis through in vitro and in vivo experiments.MethodsWe evaluated the effects of MAE on the production of nitric oxide (NO) and prostaglandin E2 (PGE2) in RAW 264.7, as well as thymus and activation-regulated chemokine (TARC/CCL17) in HaCaT cells. In an in vivo experiment, atopic dermatitis was induced by topical application of house dust mites for four weeks, and the protective effects of MAE were investigated by measuring the severity of the skin reaction on the back and ears, the plasma levels of immunoglobulin E (IgE) and histamine, and histopathological changes in the skin on the back and ears.ResultsMAE suppressed the production of NO and PGE2 in RAW 264.7 cells, as well as TARC in HaCaT cells, in a dose-dependent manner. MAE treatment of NC/Nga mice reduced the severity of dermatitis and the plasma levels of IgE and histamine. MAE also reduced the histological manifestations of atopic dermatitis-like skin lesions such as erosion, hyperplasia of the epidermis and dermis, and inflammatory cell infiltration in the skin on the back and ears.ConclusionOur results suggest that MAE has potent inhibitory effects on atopic dermatitis-like lesion and may be a beneficial natural resource for the treatment of atopic dermatitis.
Highlights
Morus alba, a medicinal plant in Asia, has been used traditionally to treat diabetes mellitus and hypoglycemia
M. alba extract (MAE) inhibits nitrite and prostaglandin E2 (PGE2) production in LPS-stimulated RAW264.7 cells The cytotoxicity of MAE was determined in RAW 264.7 and HaCaT, subsequent experiments were performed in cells at non-toxic concentrations (Figure 1)
Our study demonstrated the inhibitory effects of MAE on atopic dermatitis
Summary
A medicinal plant in Asia, has been used traditionally to treat diabetes mellitus and hypoglycemia. The effects of M. alba extract (MAE) on atopic dermatitis have not been verified scientifically. We investigated the effects of MAE on atopic dermatitis through in vitro and in vivo experiments. Atopic dermatitis is a multifactorial disease that depends on a genetic predisposition to allergy, which is characterized by complex immune mechanisms in the development of allergic skin inflammation [1]. Environmental, skin barrier, pharmacological, psychological and immunological factors contribute to the pathogenesis of atopic dermatitis, but its immunological basis is of considerable importance, and it has been studied extensively [2]. Immunological analyses of atopic dermatitis have shown that the activation of mast cells and differentiated T-helper 2 (Th2) cells by various. There was no study on the effects of M. alba on atopic dermatitis. The effect of MAE might be associated with the suppression of the inflammatory response in atopic dermatitis
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