Morphophysiology and cryopreservation of seeds of Dendrobium nobile Lindl. (Orchidaceae) at different stages of development

G L Kolomeitseva,A S Ryabchenko,T V Nikishina,A V Babosha,O N Vysotskaya

doi:10.1007/s11738-022-03372-z

Abstract

The immature seeds of Dendrobium nobile acquire resistance to freezing and long-term storage in a cryobank at the age of 6 months after pollination. Seed maturation of Dendrobium nobile Lindl to establish the optimal stage for long-term storage of immature seeds in liquid nitrogen was studied. Immature seeds can germinate in vitro starting from 3 months after pollination (MAP) but only develop up to the stage of embryo swelling or protocorm without rhizoids. The maximum staining of embryos with vital dyes FDA and TTC occurs at 4 and 5 MAP that corresponds to the release of suspensor beyond the embryo sac. Embryo staining does not correlate with germination capacity and seed viability in cryogenic storage. The immature seeds acquire resistance to drying in airflow and cryostorage at the age of 6 MAP. 11% of seeds germinated after cryogenic storage at 6 MAP, and 81–94% at 7–10 MAP.

Highlights

The ongoing interest in orchids cultivation is explained by the ornamental features of the flowers and by diverse biologically active compounds, produced in their stems and leaves
We studied the seed maturation of Dendrobium nobile Lindl to establish the optimal stage for green pod culture and long-term storage in liquid nitrogen
It was found that the immature seeds can germinate in vitro starting from 3 months after pollination (MAP) but only develop up to the stage of embryo swelling or protocorm without rhizoids

Summary

Introduction

The ongoing interest in orchids cultivation is explained by the ornamental features of the flowers and by diverse biologically active compounds, produced in their stems and leaves. We studied the seed maturation of Dendrobium nobile Lindl to establish the optimal stage for green pod culture and long-term storage in liquid nitrogen. It was found that the immature seeds can germinate in vitro starting from 3 months after pollination (MAP) but only develop up to the stage of embryo swelling or protocorm without rhizoids.

Results

Conclusion