Morphophysiological dormancy in seeds ofConvallaria keiskeiand a proposal to recognize two types of double dormancy in seed dormancy classification

Abstract

AbstractConvallariamajalishas double dormancy and hypogeal germination, but no information is available on embryo growth or on the effects of light and gibberellic acid (GA3) on germination in this genus. Therefore, we investigated embryo growth and other germination features in seeds ofC. keiskeiand compared the data with those ofTrillium camschatcensein another study. Until now, in seeds with double dormancy, embryo growth and germination (epigeal) have been studied in detail only for seeds ofT. camschatcense. Phenology of embryo growth and emergence of cotyledonary petiole/root (hereafter root) and shoot in seeds ofC. keiskeiwere monitored outdoors. Effects of temperature, light and GA3on embryo growth and root and shoot emergence were tested under laboratory conditions. Roots emerged the first spring following seed dispersal in autumn. The embryo grew soon after root emergence, and germination was hypogeal. Seeds with an emerged root formed buds from which a shoot (leaf) emerged above ground during the second spring. Alternating temperatures and light had negative effects on root emergence, and GA3did not substitute for cold stratification in root emergence. Seeds ofC. keiskeihave double dormancy, but it differs from that inT. camschatcense. Based on differences in embryo growth before (T. camschatcense) versus after (C. keiskei) root emergence, and on epigeal (T. camschatcense) versus hypogeal (C. keiskei) germination, we suggest that two types of deep simple double morphophysiological dormancy (MPD) be recognized. Since embryo growth inC. keiskeidoes not fit the standard definition of MPD, we propose to expand this definition.