Morphophysiological characterization of Ceratocystis fimbriata isolates from yerba mate

Nícolas Manarim De Brito,Celso Garcia Auer,Caroline De Bastos Bühner,Álvaro Figueredo Dos Santos,Henrique Da Silva Silveira Duarte

doi:10.1590/0103-8478cr20200579

Nícolas Manarim De Brito, Celso Garcia Auer + Show 3 more

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https://doi.org/10.1590/0103-8478cr20200579

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Abstract

ABSTRACT: This study aimed to morphologically characterize the isolates of Ceratocystis fimbriata from yerba mate and to evaluate the effect of culture medium and temperature on mycelial growth and sporulation of C. fimbriata. For the morphological characterization of the 11 monosporic isolates of the fungus, slides were prepared to determine the dimensions of the sexual and asexual structures of the fungus. Four experiments were conducted to evaluate the mycelial growth and to evaluate the sporulation of C. fimbriata in different culture mediums and temperatures. The isolates of C. fimbriata from yerba mate showed perithecia with brown to black necks, divergent ostiolar hyphae, hatshaped hyaline ascospores, single-celled, cylindrical endoconidia, and globular to ovoid aleurioconidia. PDA and V8-agar media showed the highest mycelial growth. The average optimum temperature for mycelial growth and sporulation of isolates of C. fimbriata of yerba mate were 22.5 and 22.4 ºC, respectively.

Highlights

RESUMO: Este estudo teve como objetivo caracterizar morfologicamente os isolados de Ceratocystis fimbriata e avaliar o efeito do meio de cultura e da temperatura no crescimento micelial e na esporulação de C. fimbriata
Quatro experimentos foram conduzidos para avaliar o crescimento micelial e esporulação of C. fimbriata em diferentes meios de culturae temperaturas
St. -Hil.) located in São Mateus do Sul, PR and Arvorezinha, RS, mortality of up to 5% of the plants was reported due to the wilting of yerba mate caused by Ceratocystis fimbriata Ellis & Halsted (SANTOS et al 2018)

Summary

Introduction

RESUMO: Este estudo teve como objetivo caracterizar morfologicamente os isolados de Ceratocystis fimbriata e avaliar o efeito do meio de cultura e da temperatura no crescimento micelial e na esporulação de C. fimbriata. Work, the objective was to characterize the isolates of C. fimbriata morphologically and to evaluate the effect of culture medium and temperature on mycelial growth and sporulation of C. fimbriata.

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Results

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