Morphophysiological changes in the intact surface of rat skin under the application of silicon and gold nanoparticles

Abstract

The effect of a course application of suspension of porous silicon and gold nanoparticles on the skin of female rats on the level of free radical oxidation and catalase activity in the skin homogenate was studied. MesoPSi samples were formed using the standard method of electrochemical etching of p-type c-Si wafers with a surface orientation of (100) and a specific resistance of 1-5 mOm x cm in a solution of hydrofluoric acid and ethanol (HF (50%): C2H5OH = 1: 1) at an etching current density of 60 mA / cm2 for 1 hour (45 minutes-hour). After this, mesoPSi films were separated from the silicon substrate by a short-term increase in the current density to 600 mA / cm2. Aqueous suspensions of porous silicon nanoparticles with sizes of the order of 100 nm were obtained by mechanical grinding of mesoporous silicon films in water. Aqueous suspensions of gold nanoparticles with an average size of 30-50 nm were obtained by laser ablation of gold targets in deionized water. A suspension of silicon and gold nanoparticles in physiological saline at a concentration of 0.2 mg / ml was applied once a day in the form of a thin layer on a shaved area of the skin of animals (interscapular region) and left to dry completely. Applications were carried out for 10 days. The studied silicon and gold nanoparticles did not change the initial content of malondialdehyde and catalase activity in the skin tissue but contributed to the activation of the rate of spontaneous lipid peroxidation.