Morphopatological and histochemical highlights in normal and varicose vein wall

Abstract

The nutrition of the venous wall appears to be an important factor in the vascularfibrillar trophicity and in the dynamic of the extracellular matrix formation for the normal veins and, for the chronic venous ulcers of legs, on period of healing. Sequential biopsies were taken at various levels of venous wall of external and internal saphena in 16 cases presenting a chronic condition of legs venous system (35-58 years old patients, both sexes). 8 vein fragments with normal macroscopic appearance were also taken, in necropsy. These samples were analyzed using regular morphological methods and some histochemical reactions to reveal the glycogen, glycoproteins, and glycosaminoglycans substrates. There were been used the Gomori silver impregnation and orcein to expose some specific substrates like reticulin or elastin. Other staining methods, like Gomori trichrome, were used to differentiate the specific structures of the vein wall, were used to differentiate the specific structures of the vein wall. A rich vascularization of normal and dilated vein wall could be remarked.Angiogenesis in vein wall and vasa vasorum changes as well as alcianophilic of vascular intima seem to be reactive and protective factors, depending on the applied therapeutic modalities. The veins are weak structures whose integrity depends on the thickness of the media and the support of neighboring structures.