Abstract
A method for the quantitative assessment of peptide-containing nerves in the gastrointestinal muscle layers is described. Tissue samples are sectioned obliquely, at 45°, in the plane bisecting the long axes of longitudinal and circular muscle fibres, and peptide-containing nerve fascicles are revealed by immunofluorescence. Cross-sectioned fascicles are counted (number/area of muscle) and their length density per volume of muscle layer is calculated. The composition of immunostained nerve fascicles is also assessed, by counting peptide-containing fibres in each, while peptide transmitter co-distributions are studied in parallel using adjacent tissue sections. Thus, the approach described provides a means for the delineation of both quantitative tissue distribution and composition of autonomic neuro-muscular junctions in the gastro-intestinal tract.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.