Morphometry and distribution of displaced dopaminergic cells in rat retina

Abstract

The majority of dopaminergic (DA) cells, labeled by tyrosine hydroxylase (TH) immunohistochemistry, are located in the amacrine cell layer (i.e., the innermost sublayer of the inner nuclear layer) in the rat retina. We describe a small population of DA cells, observed in retinal wholemounts, that are displaced to either the inner plexiform layer (DAIcs) or the ganglion cell layer (DAGcs). Contrary to some other species, such cells are few in number in the rat retina. Their systematic study was made in young and adult retinas by retinal mapping, camera lucida drawing, and computer-aided three-dimensional reconstruction. Located predominantly in the superior temporal quadrant, they are observed as soon as the second postnatal day. Most of the morphometric parameters studied were not significantly different between the two types of displaced DA cells, despite the characteristic appearance of interstitial cells. Two hypotheses are proposed for the origin of their displacement: either it is accidental or programmed. Our results favor the former possibility.