Abstract
Barbodes binotatus (Bleeker, 1859) is an endemic freshwater fish species that currently used for aquaculture. Good quality seed can be attained from cross-breed of parental stock from different geographical areas. This study aimed to distinguish morphological asymmetry of the species collected from three different rivers in Java: Sungai Tawang (East Java); Sungai Opak (Central Java); and Sungai Cisanggarung (West Java). Total 30 specimen were randomly sub-sampled from each river. Morphology, morphometry and meristic characters, based on 14 anatomical landmarks were measured and analyzed using Thin Plate Spline (TPSDIG), Symmetry-Asymmetry Geometric Analysis (SAGE) and Principal Component Analysis (PCA). Result of SAGE analysis showed that there were no significant asymmetries observed within, and between regions, respectively (p > 0.05). The result of PCA analysis however, showed qualitative differences in ratio between eye diameter (ED) versus head length (HL) (49%) and total length (TL) versus standard length (SL) (17%). Genetic (through DNA analysis) study need to be elaborated to see the differences more accurately.
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