Morphometric and densitometric investigations of protoplasmic astrocytes and neurons in human hepatic encephalopathy

Abstract

Morphometric and densitometric evaluations were made of nuclei of astrocytes and nerve cells of 49 cases with chronic liver diseases and of 9 control cases. The data measured from Nissl-stained specimens of putamen were compared with clinical degrees of encephalopathy and with blood ammonia levels. The parameters measured included nuclear area and optical density of nuclei. The nuclear area of astrocytes (AAREA), on average, was found to grow significantly along with aggravation of encephalopathy, that growth being from 39 micron 2 in the control group to about 60 micron 2 in cases of severe encephalopathy. Furthermore the proportion of astrocyte nuclei with an area above 70 micron 2 (ALRG) and the proportion of the optical light area of the total nuclear area (AHOLE) rises. Mean compactness (ACEXT) and mean extinction of astrocyte nuclei (AMEXT) dropped along with growing severity of encephalopathy. Mean blood ammonia levels rose from 42 mumol/l in cases with no microscopically detectable signs of encephalopathy to about six times higher values in cases with severe encephalopathy. Optical density of astrocyte nuclei was negatively correlated and mean nuclear area positively correlated to blood ammonia levels. No characteristic morphometric and densitometric changes of nerve cell nuclei were recordable from the putamen.