Morphometric analysis of collagen in gestational and retained bovine placentomes

Abstract

Bovine placentome collagen was quantified (P<0.01) at four gestational stages (90, 150, 210 and 270 d, n = 8 d ), at 2 h post partum without (n = 4) and at 2 and 12 h post partum with (n = 8) experimentally-induced placental retention. Placentome sections were fixed and stained for collagen. Fetal cotyledonary (FC) collagen volume fraction (V V) increased over days of gestation studied (V V=0.03±0.01, 0.06±0.01, 0.13±0.01 and 0.19±0.01). Fetal cotyledonary hydroxyproline (3.15±0.41, 4.55±0.41 and 7.04±0.41 mg/g) and FC protein (432.0±17.1, 479.9±17.1, 585.4±17.1 mg/g) increased over Days 90, 150 and 210 and were similar on Days 210 and 270. Fetal cotyledonary collagen V V and hydroxyproline did not differ between Day 270, retained and nonretained cotyledons. Protein concentration was higher in 2 h (578.1±18.5 mg/g) and 12 h (526.0±18.5 mg/g) retained versus nonretained (400.4±36.2 mg/g) cotyledons. Maternal caruncular (MC) collagen V V and protein concentration were higher on Days 90 and 150 than on Days 210 and 270. Maternal caruncular hydroxyproline was similar from Day 90 to 210 and increased from Day 210 to 270. Maternal caruncular collagen V V, hydroxyproline and protein concentrations were similar on Day 270 and in 2 h and 12 h retained membrane caruncles. Gestational increases in placentome collagen occurred from FC sources. No difference in FC or MC collagen V V existed between Day 270, retained and nonretained placentomes.