Morphometric analysis and genetic diversity in Pistacia species populations using sequence related amplified polymorphism

Abstract

The Anacardiaceae family includes 11 or more species, including the genus Pistacia. Pistacia vera L. is the most commercially developed of them all, thanks to its tasty nut seeds. Pistacia khinjuk Stocks, Pistacia atlantica Dsef, Pistacia vera, Pistacia eurycarpa Yalt. (Pistacia atlantica subsp. Kurdica Zoh.) and all have their origins in Iran. The present study aimed to investigate the SRAP (Sequence-related amplified polymorphism) markers in 13 wild pistachio accessions, which comprised three different species: Pistacia khinjuk, Pistacia vera, and Pistacia atlantica. Through polymerase chain reaction amplifications (PCR) of three Pistacia species, a total of 170 (Number of total loci) (NTL) DNA bands were obtained. Ten different selective primers were combined to generate these bands. The number of amplified pieces ranged from nine to twenty-six. The projected impartial gene diversity (UHe) ranged from 0.053 (Pistacia khinjuk) to 0.417 (Pistacia khinjuk) (Pistacia atlantica subsp. Kurdica). The genetic similarity of three species is estimated to be between 0.61 to 0.90. Two significant groupings emerged from the clustering findings: Pistacia khinjuk and Pistacia atlantica subsp. Kurdica exhibited the least similarity in the SRAP markers study. Our findings revealed excellent molecular recognition of all genotypes tested, indicating that a significant amount of genetic variety exists among pistachio accessions. This discovery might be useful in breeding management techniques for genetic preservation and cultivar improvement.