Morphometric analysis and genetic diversity in Hedera L. (Araliaceae) species populations using sequence related amplified polymorphism

Abstract

In temperate latitudes across Europe, North Africa, and Asia, the genus Hedera L. colonizes forest understory and riparian vegetation. The goal of this research was to investigate SRAP (Sequence-related enhanced polymorphism) markers in overall 70 successions of Hedera types, that is comprised of three species Hedera helix L.; Hedera colchica (K.Koch) K.Koch and Hedera pastuchovii Woronow. Overall, 76 (Number of total loci) (NTL) DNA bands were created via polymerase chain reaction amplifications (PCR) amplification of three Hedera types. These bands were obtained by combining five distinct selective primers. The overall amount of amplified varied from 10 to 18. The projected impartial gene diversity (UHe) ranged from 0.013 (Hedera colchica) to 0.34 (Hedera colchica) (Hedera helix). The genetic similarity of three varieties is considered to be between 0.63 to 0.90. The clustering findings revealed two main groups. Hedera helix and Hedera colchica have the least affinity in the SRAP (Sequence-related amplified polymorphism) indicators study. Our findings revealed excellent molecular recognition of every genotypes tested, indicating that the Hedera accessions exhibit a lot of genetic variation. This discovery might be useful in breeding control techniques for genetic preservation and cultivar growth.