Morphology-Controllable Collagen/Poly(2-hydroxyethyl methacrylate) Porous Hydrogel with a Paraffin Microsphere as a Template.

Abstract

In this study, a porous poly(2-hydroxyethyl methacrylate) (PHEMA) matrix was fabricated by a paraffin template method, which was used as a substrate to adhere collagen fibers to form an interconnective porous collagen/PHEMA (Col-PHEMA) composite hydrogel. A microscope and scanning electron microscope (SEM) were employed to characterize the morphology of paraffin microspheres and Col-PHEMA composite hydrogels. The paraffin microspheres with the diameter in the range from 100 to 200 μm were collected by a preset sieve. Then, the interface of uniform paraffin microspheres were thermally bonded to form a contacted template, and the derived Col-PHEMA composite hydrogels had an interconnective porous microstructure. Fourier transform infrared spectroscopy (FTIR) indicated that new hydrogen bonds were formed between collagen fibers and the PHEMA hydrogel. Besides, the Col-PHEMA composite hydrogels revealed a high hydrophilicity, good mechanical properties, and good water uptake capacity. The porous Col-PHEMA composite hydrogels showed a good biocompatibility, and the collagen layer may promote the proliferation of fibroblast cells. The Col-PHEMA composite hydrogel is expected to find an application in corneal repairing.