Morphology, Phylogeny and Pathogenicity of Colletotrichummenglaense sp. nov., Isolated from Air in China.

Min Qiao,Lin-Lin Fang,Jian-Ying Li,Ze-Fen Yu,Jie Li

doi:10.3390/pathogens10101243

Min Qiao, Lin-Lin Fang + Show 3 more

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https://doi.org/10.3390/pathogens10101243

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Abstract

A new species, Colletotrichum menglaense, isolated from air in Mengla, Xishuangbanna, Yunnan Province, China, was characterized and described combining morphological characteristics and multigene phylogenetic analysis. Morphologically, it is characterized by oblong, sometimes slightly constricted, micro-guttulate conidia and simple obovoid to ellipsoidal appressoria. Phylogenetic analysis of the ITS, ACT, CHS, and GAPDH sequences showed that C. menglaense belongs to the C. gloeosporioides complex. The pathogenicity of C. menglaense on fruits of several crop plants, including strawberry, orange, grape, tomato, and blueberry, was tested and confirmed by the re-isolation of C. menglaense.

Highlights

There are numerous bacteria and fungi in the near-surface atmosphere and upper troposphere, with millions of cells per cubic metre of air [1]
When we investigated the fungi diversity of air samples in the town of Mengla, Xishuangbanna, a new species of Colletotrichum was identified based on morphological characteristics and DNA sequence data from four loci, and we named it C. menglaense
In the phylogenetic tree inferred from internal transcribed spacer (ITS), the strain is well clustered in the Colletotrichum gloeosporioides complex

Summary

Introduction

There are numerous bacteria and fungi in the near-surface atmosphere and upper troposphere, with millions of cells per cubic metre of air [1]. Many investigations recovered fungi from various places: the atmosphere in the urban environment of the USA [19], a rural area in India [20], at an altitude of 20,000 m in Earth’s atmosphere [5], in southeastern Austria [16], and from the Himalayan region [21]. Most of these studies monitored remote, extreme, sparsely populated sites to learn the diversity, distribution, and seasonal variations of fungal species [22]. Benj [13], and occasionally species of Colletotrichum Corda [24,25]

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