Abstract
Pleomorphic cells were observed in cultures of Vibrio cholerae incubated under anaerobic conditions. The appearance of these pleomorphic cells coincided with the detection of large amounts of cholera enterotoxin in the culture filtrates. Many of these pleomorphic cells had morphologically defective cell envelopes which appeared to lack a murein layer. They also contained intracellular membrane-bounded vesicles. The nature of these membrane structures and their association with enterotoxin production is discussed.
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