Abstract
Tumours in hamsters, induced by the chicken-embryo-lethal-orphan (CELO) virus, by tumour tissue transplants, or by tumour cells grown in culture, were well circumscribed solid tumours and covered by a thin capsule-like structure. All were fibrosarcomata. However, tumours produced by the 3 inocula exhibited the following histological differences. Neoplasms induced by CELO virus were generally less differentiated and were composed of cells with polygonal or oval nuclei and indistinct cytoplasmic boundaries. Numerous multinucleated bizarre giant cells were found. Those produced by tumour tissue transplants were more differentiated and were composed of spindle shaped cells with abundant collagen fibre formation. Neoplasms induced by tumour cells grown in culture were generally undifferentiated with many mitotic figures and contained numerous giant cells.Cells from tumours induced by CELO virus or tumour transplants produced similar morphologies when cultured in vitro. The cell cultures consisted of large cells with oval or rounded large nuclei and prominent nucleoli. Multinucleated giant cells, cells in mitosis, and a disorganized growth pattern were also characteristic of the cell cultures. However, mitosis and a piling-up of cells occurred more frequently with cell cultures derived from the CELO virus-induced tumour.
Highlights
Summary.-Tumours in hamsters, induced by the chicken-embryo-lethal-orphan (CELO) virus, by tumour tissue transplants, or by tumour cells grown in culture, were well circumscribed solid tumours and covered by a thin capsule -like structure
This paper is concerned with the comparison of morphologies of tumours induced in hamsters by CELO virus, by CELO-induced tumour tissue, and by cells from cell cultures derived from a primary CELO-induced tumour and from a CELO-transplant-induced tumour
The transplanted tumour tissue was derived from neoplasms induced with CELO virus, cells from the T5 or T37 cell cultures, or from transplanted tumour tissue of CELO-induced neoplasms
Summary
CELO virus (Phelps strain), isolated in April 1954 (Yates and Fry, 1957) and since stored at -20°C, was thawed and passed 3 times in 10-day-old embryonated chicken eggs. The allantoamniotic fluids from the third passage were harvested and pooled. This pool of virus was dispensed in 1 ml aliquots and stored at -20°C. It possessed an ELD50 titre of 109 2/ml
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