Morphology of thalamocortical neurons projecting to the primary somatosensory cortex and their relationship to spinothalamic terminals in the squirrel monkey.

Abstract

This study examined the morphology of thalamocortical neurons projecting to the primary somatosensory cortex (SI; hand region of areas 3a, 3b, 1, and 2) and their relationship to the spinothalamic (STT) terminals in the squirrel monkey. Retrogradely labeled thalamocortical neurons were intracellularly filled with Lucifer yellow (LY), and the STT terminals were anterogradely labeled with biotinylated dextran. Both filled neurons and labeled terminals were differentially visualized in the same field by a dual immunocytochemical staining method. SI-projecting neurons appeared at the light level to be in contact with STT terminal boutons in the ventroposterior lateral (VPL), ventroposterior inferior (VPI), and centrolateral (CL) nuclei and the posterior complex (PO). The analyses of the neuronal morphology revealed that somatic and dendritic morphologies of SI-projecting neurons in these thalamic nuclei, as well as in the anterior pulvinlar (Pulo), centromedial (CM), and ventrolateral (VL) nuclei, were generally comparable with some exceptions: VL neurons had the largest soma sizes, the most primary dendrites, and the longest total dendritic length among all neurons studied; VPI neurons had the smallest soma sizes; VPL SI-projecting neurons were different from those in VPI in their soma sizes, shape factors, and orientations; in VPL the cells projecting to the superficial layers of SI were smaller than those projecting to the deeper layers, but in VPI the two groups of neurons were similar in soma sizes. In general, the SI-projecting neurons in VPL, VPI, and CL were similar in their dendritic morphologies and branching patterns, and varied from those in Pulo, PO, CM, and VL.