Morphology of Gushu decoction in rabbit model of knee osteoarthritis study observation and influence of BFGF expression

Abstract

Purpose: This topic seeks to under the guidance of TCM theory, combined with modern scientific research methods, through the rabbit knee osteoarthritis experimental and clinical research, observation of compound Chinese medicine Gushu decoction right knee osteoarthritis rabbit model of morphology and bFGF expression, and to explore the mechanism of therapeutic efficacy of KOA, and to further lay the foundation for clinical application and promotion. Methods: 48 healthy adult Japanese white rabbits were randomly divided into four groups: A Blank group, B model group, C bolus group, D Gushu decoction groups of 12 rats, each weighing mark. In addition to the blank group are used outside the Hulth method modeling, cut off anterior cruciate ligament, pay attention not to damage articular cartilage. Modeling is successful, A group and B group were fed with 10ml of distilled water, without other ingredients; C group were given bolus 10ml (0.7g/ml) aqueous gavage, D group were given Gushu decoction 10ml (0.7g / ml) aqueous gavage. Continuous administration of 8 weeks, rabbits were sacrificed in each group,Take synovial, articular cartilage specimens HE staining under light microscope morphology and rickets reason score, using immunohistochemistry to detect the expression of bFGF in articular cartilage. Results: The Gushu decoction can significantly increase the expression of bFGF in articular cartilage. The results showed that the apparent model group, synovitis of the knee joint changes and degenerative changes in cartilage; C group, D group significantly better than the model group pathological score (P <0.01); C group, D group compared pathological score was significantly differences. Postoperative phases of gray values were significantly lower than model group (P<0.01), shows that C group, D group could significantly up-regulated bFGF expression. The naked eye and optical microscope observation C group,D group synovial and the pathological changes of articular cartilage significantly lighter than the model group. Conclusions: The Gushu decoction may be through up-regulating the expression of bFGF in articular cartilage and improve the pathological score, thus inhibiting the release of inflammatory mediators, reducing synovitis disease, to improve the function of chondrocytes and delay the degeneration of articular cartilage to protect the role of articular cartilage, For osteoarthritis and thus play a certain therapeutic effect.View Large Image Figure ViewerDownload Hi-res image Download (PPT)View Large Image Figure ViewerDownload Hi-res image Download (PPT)View Large Image Figure ViewerDownload Hi-res image Download (PPT)