Morphology of basal optic tract terminals in the turtle, Pseudemys scripta elegans.

Abstract

The morphologies of axon terminals of retinal ganglion cells projecting to the basal optic nucleus (BON) via the basal optic tract (BOT) were studied in the red-eared turtle. The BOT was visualized on the ventral surface of the brainstem in vitro, and either biotinylated dextran amine was injected extracellularly or neurobiotin was injected into physiologically identified axons during intracellular recordings. Up to 16 hours after tracer injection, the brains were fixed, sectioned parasagittally, and stained for biotin and Nissl substance. The diameters and depths of extracellularly filled axons were measured at three BON sites. Fourteen axons were reconstructed from serial sections with the aid of appropriate computer software. Analysis of extracellularly filled retinal axons revealed that about three times more axons were present just inside the rostral border of the BON compared with its caudal border. Thick (2-4 microm) axons were located within 100 microm from the ventral border, whereas thin (<2 microm) axons were found throughout the nucleus. Only the thinnest axons (<1 microm) extended caudally from the nucleus, indicating that some extracellularly labelled fibers passed through the BON. The intracellularly filled axons were more similar to the thick axons filled extracellularly and arborized entirely within the BON. All of the intracellularly filled axons had thick ventral trunks from which many thin branches extended dorsally and obliquely within the BON. The thin branches bifurcated repeatedly to form bead-like varicosities or boutons that often formed clusters within regions of 150 microm3 or less. These clusters may reflect areas of focused synaptic contact on BON cells with specific direction preferences.