Morphology, morphogenesis and molecular phylogeny of a novel saline soil ciliate, Lamtostyla salina n. sp. (Ciliophora, Hypotricha)

Abstract

The morphology and morphogenesis of a new saline soil hypotrich, Lamtostyla salina n. sp., collected from Longfeng Wetland in Daqing, north China, were studied based on live observations and protargol stained specimens. The new species is characterized as follows: body very flexible but not contractile, lanceolate with anterior end broadly rounded, widest at about 1/3 of body length, posterior end narrowly rounded; cortical granules colourless and scattered; amphisiellid median cirral row ends ahead of mid-body, composed of 5–13 cirri; 4–20 frontoventral cirri arranged in 2–4 rows; three frontal, one buccal and 2–6 transverse cirri; usually one left and one right marginal row, composed of 17–51 and 20–51 cirri respectively; usually two dorsal kineties; two macronuclear nodules and two micronuclei. Morphogenesis is typical of the genus Lamtostyla: parental structures are involved in the formation of frontoventral transverse anlagen in the proter. Phylogenetic analyses based on small subunit ribosomal DNA sequence data reveal that the systematic position of Lamtostyla is rather unstable with low support values across the tree. However, a well-recognized close relationship between Lamtostyla and Bistichella are shown in all phylogenetic analyses.