Morphology and Physiology of Lyngbya nigra with Reference to Copper Toxicity

Abstract

AbstractThe effect of copper sulphate on morphology and physiology of Lyngbya nigra has been studied. The growth was inhibited in all treatments (0.4 to 80.0 μM) of copper sulphate. There were no apparent morphological changes up to 0.8 μM and during the first two days of treatment even in the higher concentrations of copper sulphate.In concentrations above 0.8 μM the first symptom of toxicity was the formation of separation discs in large numbers. The trichomes contracted longitudinally and the cells became swollen and constricted at the cross walls. The cells also became yellowish due to loss of photosynthetic pigments. Finally, in 4 μM and above, vacuoles appeared in large number indicating the moribund state of the cells.Copper sulphate increased respiration at 2 μM, and optimum effect was observed in 8 μM after 96 h. Inhibition of photosynthesis was detectable in 0.8 μM, and 100% inhibition took place in 8 μM after 96 h. In higher concentrations the effect was immediate, and a conspicuous inhibition of photosynthesis could be observed within 10 min.The copper content of the alga increased with increased concentration of copper sulphate while potassium content decreased. With rise in outside concentrations of copper, there was a comparatively great increase of absorption in 2 and 4 μM, while further increases were gradually less. The observations indicate that changes in the physiological activity of the alga under treatment are closely interlinked with marked changes in morphology.