Morphology and molecular phylogeny of two marine diatom species, Falcula cf. media and F. rectangularis sp. nov. (Bacillariophyta) from Japan

Abstract

ABSTRACT Two members of the marine diatom genus Falcula, which has been little known for a long time, were found from the surface of some Japanese seaweeds; one was assigned to F. cf. media and the other was described as a new species, F. rectangularis sp. nov. Aside from the arcuate valves and slit-like apical fields, typical of the genus, detailed morphological observations using light and scanning electron microscopy revealed resemblance between F. cf. media and F. rectangularis in areolae, stria density and lack of costae. However, the two species can be distinguished based on several morphological features, including the valve length, plastid arrangement, and frustules in girdle view. A three-gene molecular phylogeny recovered these two species as monophyletic with a high interspecific rbcL difference, which in turn were sister to the clade containing taxa that belong to the orders Cyclophorales and Protoraphidales with low support. The members in this poorly supported assemblage share apical fields composed of slits. The two species of Falcula were phylogenetically distinct from Pseudofalcula hyalina, a member previously assigned to Falcula.