Abstract
The freeze-fracturing of purified dengue-2 virus has shown at least three distinct fracture planes: a grazing fracture removing at most some surface projections; a fracture apparently splitting the membrane; and a cross fracture through the nucleocapsid of the virus. Morphogenesis in LLC-MK2 cell culture was studied by use of the freeze-fracture technique and also by thin sectioning. Freeze-fracturing has been helpful in determining the sites and extent of virus development before the virus appears in vacuoles, and in determining related phenomena of infection. There is no direct evidence from freeze-fracturing for the “budding” of virus into these vacuoles, and their release by reverse pinocytosis, both of which have been previously proposed. However, evidence from thin sections herein suggests that release of mature virus from the cell may yet be by a reverse pinocytosis from small vesicles involved with the normal mechanisms of intracellular transport.
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