Abstract
One thirty samples (fifty-five potato tubers, twenty-seven potato stems, three chili stems, twenty-eight soil samples, five weed samples, three banana leaves, and nine water samples) were examined and one hundred six (106) Bangladeshi isolates of Ralstonia solanacearum were isolated and identified. Isolation was made on selective media (Tetrazolium chloride media) and R. solanacearum was identified based on morphological, pathological and biochemical properties and polymerase chain reaction (PCR) by using the species-specific primers. Studies showed that 81.54% (106) samples were positive on tetrazolium chloride solid medium. Among them 90 isolates were virulent and rest of them were avirulent. Fifty isolates were selected for chemical characterization based on hypersensitivity test. R. solanacearum is gram negative, aerobic facultative bacteria on the basis of chemical characterization. Fifty tested isolates expressed as race 3 while in biovar test forty-eight showed as biovar III and the rest two showed as biovar I. In nine tested isolates from the three districts a species-specific band of 280 bp was amplified in PCR that confirmed the identity of R. solanacerum.
Highlights
R. solanacearum [1] formerly called Pseudomonas solanacearum [2] is the most destructive, damaging soil-borne pathogen [3] [4] [5]
Isolation was made on selective media (Tetrazolium chloride media) and R. solanacearum was identified based on morphological, pathological and biochemical properties and polymerase chain reaction (PCR) by using the species-specific primers
It was observed that twenty-seven potato plant samples showed positive bacterial ooze streaming in clear water but three chilli samples showed negetive in streaming test (Table 3)
Summary
R. solanacearum [1] formerly called Pseudomonas solanacearum [2] is the most destructive, damaging soil-borne pathogen [3] [4] [5]. It is very destructive pathogen of potatoes in temperate, subtropical and tropical regions throughout the world [9] [10]. R. solanacearum is gram-negative, rod-shaped bacterium measuring 0.5 - 0.7 × 1.5 - 2.0 μm in size. This pathogen is severed at 24 ̊C - 35 ̊C temperature but optimum at 28 ̊C - 32 ̊C in aerobic conditions [11] [12]. Ralstonia solanacearum caused bacterial wilt, southern wilt and brown rot of potato world wide. Infection of potato tubers with R. solanacearum may become latent under conducive environmental conditions [13]
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