Morphological function of toe fringe in the sand lizard Phrynocephalus mystaceus

Peng Zheng,Tao Liang,Lei Shi,Jing An

doi:10.1038/s41598-020-79113-4

Peng Zheng, Tao Liang + Show 2 more

Open Access

https://doi.org/10.1038/s41598-020-79113-4

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Abstract

Toe fringe is the most typical morphological feature of lizards adapted to sandy environments, and it is simple in shape, can evolve repeatedly, and has a high degree of repetition; therefore, this feature is suitable for testing the adaptive convergence suggested by form-environment correlations. Phrynocephalus mystaceus mainly lives in dune habitats, has a developed bilateral toe fringe, and exhibits fast sand-burying behavior for predator avoidance. We tested the effects of resecting the medial and bilateral toe fringes on the locomotor performance and sand-burying performance of P. mystaceus. The results showed that the maximum sprint speed and acceleration on sand substrate did not significantly differ under different conditions (P > 0.05). Sand-burying performance scores of the unresected individuals were significantly greater than those of the resected individuals (P < 0.05). A partial least squares (PLS) regression analysis showed that the relative area of toe fringe was the main factor affecting the sand-burying performance of unresected P. mystaceus. For lizards without fringe, the PLS regression showed that the swinging index of the hind-limb was the main factor affecting the sand-burying performance of the lizard. A comparison of the swinging indexes of the hind-limb of the lizard under three states revealed that under the unresected states, the frequency of the swinging of the hind-limb was significantly higher than those of lizards with resected bilateral fringes, further indicating that the lizards compensated for the loss of fringe by increasing the time and frequency of swinging of the hind-limb. A path analysis also showed that the fringe affected the sand-burying performance of P. mystaceus not only directly but also indirectly by affecting the frequency of the swinging of the hind-limb. After the bilateral toe fringe was removed, a significant negative correlation between locomotor and sand-burying performance was observed (P < 0.05). Taken together, these results provide experimental evidence that toe fringe is positively associated with the sand-burying performance of P. mystaceus.

Highlights

Locomotion is a fundamental component of prey capture by and evasion of p redators[1], and an animal’s escape behavior should reflect both the cost of interrupting current activities to respond to predators and the relative risk of predation[2,3]
The mass was recorded on an electronic balance to the nearest 0.01 g, and the length of the forelimb and the hind-limb was divided into two parts: the humeral length (HL1) and radius length (RL) and femur length (FL) and tibia length (TL1)
The partial least squares (PLS) regression analysis showed that under the uncut and double-cut states, due to the weak correlation between the maximum sprint speed and morphological characteristics, none of the morphological characteristics were the major determinants of locomotor performance

Summary

Introduction

Locomotion is a fundamental component of prey capture by and evasion of p redators[1], and an animal’s escape behavior should reflect both the cost of interrupting current activities to respond to predators and the relative risk of predation[2,3]. C. draconoides and U. scoparia belong to different genera and show great differences in the relative limb proportions and potential behavioral, ecological, and physiological aspects These variations complicate the examination of interspecific p erformance[12]. We measured several morphological traits and analyzed the locomotor and sand-burying performance of P. mystaceus on sand substrates. We adopted a control test that consisted of removing the toe fringe to verify the following scientific hypotheses: (1) The presence or absence of toe fringe on P. mystaceus will affect its locomotor performance over sand substrates. (2) The presence or absence of toe fringe on P. mystaceus will affect its sand-burying performance on sand substrates. (3) The toe fringe of P. mystaceus will influence the locomotor and sand-burying performance, and a trade-off occurs between the two performance levels We adopted a control test that consisted of removing the toe fringe to verify the following scientific hypotheses: (1) The presence or absence of toe fringe on P. mystaceus will affect its locomotor performance over sand substrates. (2) The presence or absence of toe fringe on P. mystaceus will affect its sand-burying performance on sand substrates. (3) The toe fringe of P. mystaceus will influence the locomotor and sand-burying performance, and a trade-off occurs between the two performance levels

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