Morphological features of coilocytary atypism of squamous papillomas of dogs

Natalia Kudacheva,D Fayzrakhmanov,R Shaydullin,F Nezhmetdinova,B Ziganshin

doi:10.1051/bioconf/20201700051

Natalia Kudacheva, D Fayzrakhmanov + Show 3 more

Open Access

https://doi.org/10.1051/bioconf/20201700051

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Abstract

Coilocytic atypism of tumors of epithelial origin is considered as a marker of HPV infection, regardless of the type of neoplasm, which is a clinical manifestation of papillomatosis. Many neoplasms in dogs are diagnosed depending on the identification of specific cellular and tissue atypism, which underlies the histological diagnosis. According to the recent studies, the following types of neoplasm can be distinguished depending on the taxonomic affiliation of HPV: papillomas of various localization, cancerous epidermodysplasia verruciformis, pigment affects (including plaque), inverted and squamous papillomas, squamous cell carcinomas. Histological studies are an affordable diagnostic method that allows studying the morphological features of coylocytary atypism of squamous papillomas of dogs. Squamous cell papillomas in dogs have a number of morphological and cytological features, due to the predominance of the keratinization process. In this case, the process of differentiation of epithelial cells is consistent and all layers typical of the skin of dogs are distinguished. The corneal layer prevails over all the described layers, the activity of the cells of the basal layer is noted. Coilocytes are localized in a spinous and granular layer, but depending on their location, they have distinctive morphological and quantitative characteristics. Despite the polymorphism of the manifestation of coylocytary atypism in animals within the limits of specific affects, as well as depending on the type of neoplasm, there are no common morphological criteria for evaluating coylocytosis depending on oncogenesis. It was noted that the morphological and quantitative characteristics of coilocytes depend on their location, while they have a morphology described by many researchers, including in various animal species.

Highlights

The diagnosis of animal papillomatosis is mainly focused on the identification of certain histological informative criteria characteristic of neoplasms in accordance with the morphological classification of tumors [1]
Exophytic papillomas, including squamous papillomas, endophytic inverted papillomas, pigmented plaques and invasive squamous cell carcinomas of dogs are associated with HPV, which goes beyond the causal relationship of the virus only with oral papillomatosis [4, 5]
Neoplasms are characterized by exophytic growth, different localization; the surface imitates the structure of the skin, which allows them to be referred to tumors of epithelial origin during visual examination (Fig. 1)

Summary

Introduction

The diagnosis of animal papillomatosis is mainly focused on the identification of certain histological informative criteria characteristic of neoplasms in accordance with the morphological classification of tumors [1]. Histological analysis is a differential diagnostic method that allows identifying epithelial neoplasms with oncogenic potential [2, 3]. A morphological criterion for epithelial affects in papillomatosis is considered to be coilocyte, a cell of the intermediate or superficial layer of a stratified squamous epithelium with discariosis and a perinuclear “enlightenment” zone [6]. Histological studies reveal other specific morphological features, in particular different degrees of hyperkeratosis or parakeratosis, proliferation of squamous epithelium, in the form of multiple finger-shaped formations [8, 9]

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