MORPHOLOGICAL DOMINANCE OF ISOLATES AT TWO SITES WITH LESS HUMANOID INTERFERENCE IN MAHARASHTRA, INDIA

Abstract

Products of biological origin are highly demanded in the industrial sector and because of the substantial financial gains made in the enzyme industry; more and more of these businesses are springing up. This study presents the results of processing samples collected in areas with less human interference, such as Jhow Island and the Borivali Monari Creeks. The samples were collected under the supervision of a forest officer after receiving permission from the Mangrove Cell in Maharashtra. Isolate morphology was investigated in this study. Forty-two isolates from the Borivali site shared the following characteristics: circular shape, entire margins, convex, small size colonies, smooth textures, cream pigments, opaqueness, gram positivity, and a major organism group belonging to Coccus. Twenty isolates were collected from Jhow Island, and their predominant features were as follows: circular shape, entire margins, flat elevation, punctiform colonies, smooth textures, tan pigments, opaqueness, Gram positivity, and the organism's major group belonging to Coccus. Protease, amylase, and cellulase were screened for first because of their vital role in industry. Protease producers were chosen for further testing, and using the inverted pyramid technique, the highest protease-producing isolate, Bor S17B13, was chosen for enzymatic activity. 16S rRNA sequencing was used to determine the identity of isolate Bor S17B13, and a phylogenetic tree was constructed to show that Bor S17B13 is a member of the Priestia aryabhattai strain. The gene sequence for Priestia megaterium strain B21 can be found in the National Center for Biotechnology Information database under the accession number OM743775. Protease enzymes can be used for anything from bio-industry to environmental cleanup (bio-remediation). New possibilities for scaling up enzyme production will become available as more research is done.