Abstract
Explants of neonatal mouse hippocampus cultured for up to 65 days were studied by Bodian's silver method and electron microscopy. Two principal types of neurons usually found in vivo, the hippocampal pyramidal cells and the dentate granule cells, were identified in the cultured explants by these techniques. Electron microscopic observations demonstrate that the fine structure of neurons and synapses found in the explants faithfully corresponds to that of mature neurons in vivo. Besides axosomatic and axodendritic synapses, the presence of large complex axonal endings, which are probably those of mossy fibers, is also demonstrated and the morphological features of oligodendrocytes and astrocytes are described.
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