Abstract
Mosquito Ae. aegypti is the main dengue virus vector which causes the virus to grow and develop properly. The Artificial Membrane Feeding (AMF) method is a method of indirectly transmitting the dengue virus in the Ae. aegypti. This method uses blood feed with EDTA anticoagulant and DEN-3 virus in the insectarium. One way to detect the dengue virus found in Ae mosquito cells. aegypti is an immunohistochemical Streptavidin Biotin Peroxidase Complex (SBPC). In this method, the morphological picture of DEN-3 virus infection cells will be seen through the heparin anticoagulant blood feed using the AMF method. Anticoagulants function to slow the blood clotting to feed the Ae mosquitoes. Aegypti. The aim of this study was to determine the Positive Infection Rate of DEN-3 virus and the morphological picture of DEN-3 virus infection cells using the AMF method of EDTA anticoagulant blood feed through SBPC immunocytochemical examination of Ae mosquitoes. aegypti. The design method of this study was experimental, infected Ae.aegypti mosquitoes using anticoagulant blood containing DEN-3 virus orally as an infectious sample. Negative control used Culex Sp mosquitoes and positive control used adult Ae.aegypti mosquitoes which were infected with DEN-3 virus by injection. Detection of DEN-3 virus in Ae.aegypti mosquitoes through SBPC immunositochemical examination. Results of Positive Infection Rate for Ae.aegypti mosquitoes with EDTA anticoagulant as much as 23.20% through SBPC immunocytochemical examination with a picture of cell morphology of DEN-3 virus infection. The conclusion of SBPC immunositochemical examination showed positive cell morphology of DEN-3 virus infection with EDTA anticoagulant in Ae.aegypti mosquitoes fed by human blood orally through the AMF method.
Highlights
Dengue Haemorrhagic Fever (DHF) is a disease caused by dengue virus consisting of 4 virus serotypes, DENV-1, DENV-2, DENV-3 and DENV-4
These results prove that the EDTA anticoagulant produces a Positive Infection Rate (PIR) of Ae. aegypti through
The results of clinical analysis tests on the Positive Infection Rate (PIR) of Ae. aegypti with anticoagulant using the Immunocytochemistry method, there was a significant difference. These results can be seen from the average ranking between groups so that it is proven that EDTA can be used as an anticoagulant in developing the DEN-3 virus in the body cells of Ae mosquitoes. aegypti
Summary
Dengue Haemorrhagic Fever (DHF) is a disease caused by dengue virus consisting of 4 virus serotypes, DENV-1, DENV-2, DENV-3 and DENV-4. Arthropod-Borne Virus, Flavivirus genus, and Flaviviridae family, and is transmitted through the bite of Aedes aegypti (Ae. aegypti) and Aedes albopictus (Ae. albopictus). 1. The World Health Organization (WHO) noted as Indonesia the country with the highest dengue fever case in Southeast Asia since 1968 until 2009 and has increased the number of DHF cases from 58 cases in 1968 to 126,675 cases in 2015 2.3. The high rate of dengue fever mortality causes interest for researchers to know the role of Ae. aegypti mosquitoes as a research model and can be reared in the.
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