Abstract
Highly purified preparations of equine herpesvirus type 1 virions were examined in the electron microscope after preparation by freeze etching, freeze fracturing, critical point drying, and conventional negative staining methods. Virion envelopes were covered with 12- to 18-nm particles and contained intramembranous particles whose distribution was different from those detected in envelopes of herpes simplex virus type 1 ( Rodriguez and DuBois-Dalcq, 1978 ). Observation of virions and deenveloped virus indicated that the tegument was a defined layer of material attached to the capsid, probably at the vertices. Tegument adhered to deenveloped virus, and its morphology was dependent upon the method of preparation for electron microscopy.
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