Abstract
A human malignant continuous cell line, named NG97, was recently established in our laboratory. This cell line has been serially subcultured over 100 times in standard culture media presenting no sign of cell senescence. The NG97 cell line has a doubling time of about 24 h. Immunocytochemical analysis of glial markers demonstrated that cells are positive for glial fibrillary acidic protein (GFAP) and S-100 protein, and negative for vimentin. Under phase-contrast microscope, cultures of NG97 showed cells with variable morphological features, such as small rounded cells, fusiform cells (fibroblastic-like cells), and dendritic-like cells. However, at confluence just small rounded and fusiform cells can be observed. At scanning electron microscopy (SEM) small rounded cells showed heterogeneous microextentions, including blebs and filopodia. Dendritic-like cells were flat and presented extensive prolongations, making several contacts with small rounded cells, while fusiform cells presented their surfaces dominated by microvilli.We believe that the knowledge about NG97 cell line may be useful for a deeper understanding of biological and immunological characteristics of gliomas.
Highlights
Malignant gliomas are the most common type of brain tumor in adults
Markers Immunocytochemical analysis of glial markers in the NG97 cells demonstrated that a large number of cells were
PFhigausereco2ntrast micrographs of NG97 cells Phase contrast micrographs of NG97 cells. (A) small rounded cells growing as floating aggregates; (B) dendritic-like cells appear in the culture (→); (C) a dendritic-like cell with an extensive cytoplasmatic prolongation (→) and extra numerary nucleous (Ј); (D) confluent monolayer with small rounded and fibroblastic-like cells
Summary
Malignant gliomas are the most common type of brain tumor in adults. These tumors are highly invasive and despite multi-modality treatment the mean survival of patients is still less than 1 year.Cultures of malignant cells represent an excellent and permanent material for studying the biology of these tumors as, for example, specific antigens characterization, bioactive factors produced, determination of cellular proliferation, and heterogeneity of genotypic and phenotypic characteristics (Pohl et al 1999; Tsujino et al 1997; Bodmer et al 1989; Di Tomaso et al 2000; Halfter et al 1998; Bigner et al 1981).Recently, we have established a human glioma cell line from tissue obtained from a patient diagnosed with glioblastoma multiforme of the right temporal lobe. Malignant gliomas are the most common type of brain tumor in adults. These tumors are highly invasive and despite multi-modality treatment the mean survival of patients is still less than 1 year. We have established a human glioma cell line from tissue obtained from a patient diagnosed with glioblastoma multiforme of the right temporal lobe. Histological examination revealed a grade III astrocytoma according to the WHO classification. This cell line, called (page number not for citation purposes)
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