MORPHOLOGICAL CHARACTERISTIC OF THE SPLEEN AS A HEMOLYMPHOID ORGAN WHEN INVADED BY OPISTHORCHIS FELINEUS (RIVOLTA)

Abstract

For the first time, we have studied the morphological picture of changes occurred in the spleen during invasion by Opisthorchis felineus (Rivolta), which was simulated in large rodents (rabbits). The experiment was performed on 16 animals infected orally with 50 metacercaria each. Histological sections of the spleen were made and stained with hematoxylin-eosin and by Van Gieson’s stain. Histological preparations were studied by the light microscopy method with morphography and morphometry, and the data obtained were processed statistically. There were morphological characters found of moderate stromal fibrosis, as well as infiltration of the mantle zone of lymphoid follicles by mast cells with partially degranulated cytoplasm, and areactive follicles as related to reactive ones which were exceeded by three times. Homogeneous islands of oxyphilic substance were found in the stroma. Thus, in the course of the experiment, morphological changes were found in the spleen invaded by Opisthorchis felineus (Rivolta), which were manifested in the organ fibrosis due to the moderate collagenogenesis through active fibroblasts. The lymphoid tissue of the white pulpa was characterized by inhibited formation of germinal centers in the most lymphoid follicles. The stromal component reconstruction with the displacement of lymphoid tissue was noted. Mast cells in the white pulpa characterize inflammatory and allergic reactions.