Morphological changes of Candida albicans induced by micafungin (FK463), a water-soluble echinocandin-like lipopeptide.

Abstract

Micafungin (FK463), a novel water-soluble echinocandin-like lipopeptide, exerted fungicidal action toward growing cells of Candida albicans at concentrations of 0.1 microg ml(-1) or above. The drug at these levels induced osmotically fragile cells and the resulting fungicidal effect was partially reversed when cultures were grown in the presence of an osmotic stabilizer. Candida cells incubated with fungicidal concentrations of micafungin gradually increased in size and/or became deformed. An electron microscopic study of such micafungin-treated cells revealed morphological alterations in the cell wall; deformation in contour, abnormal septum formation and decrease in thickness of the intermediate layer of the cell wall were prominent. In addition, the structure of cell membranes as well as of membranous cytoplasmic organelles was slightly impaired. These data suggest that micafungin principally affects the normal formation of the cell wall in growing Candida cells.