Morphological changes in the masseter muscle and its motoneurons during postnatal development.

Abstract

It has been suggested that the morphological properties of the masseter muscle are changed by the masticatory activity pattern. In the rat, the activity pattern of the muscle alters from sucking to biting around 3 weeks after birth. The working hypothesis in this study is that the unique alteration in masticatory activity has an important influence on the development of the masseter muscle and its motoneurons. We examined the morphological changes in the muscle fibers of the superficial masseter muscle and its motoneurons from 2 days to 280 days after birth in the rat. The change in masseter muscle activity sucking and biting was confirmed by electromyography. To label motoneurons innervating the muscle, horseradish peroxidase was injected into the muscle. The muscle and lower brain stem were sliced and processed histochemically to measure the diameters of muscle fibers and its motoneurons in the trigeminal motor nucleus. In addition, composition of myosin heavy chain (MHC) isoforms of the muscles were analyzed using gradient sodium dodecyl sulphate-polyacrylamide gel electrophoresis. There was a rapid growth in both types of muscle fibers (fast-twitch oxidative glycolytic muscle fibers and fast-twitch glycolytic fibers) for 42 days after birth, and then a gradual growth lasting until 280 days after birth. Particularly, rapid growth of the muscle fibers was seen between 21 days and 42 days after birth. A large amount of neonatal type MHC disappeared between 21 days and 42 days after birth. In the motoneuron, there was a rapid growth of motoneurons by 42 days after birth but no significant growth was seen thereafter. These results suggest that the alteration of mastication activity from sucking to biting has a significant influence on morphological development of both types of muscle fibers, but not on that of motoneurons innervating the masseter muscle.