Abstract
It is well established that ultraviolet B (UVB) radiations present in sunlight are major causes of squamous cell carcinoma (scc) in humans. Although UVB induced DNA damage in skin cells has been studied extensively, the morphological changes that follow after UVB exposure have not be examined in details. In this study mouse skin cells NIH 3T3 fibroblasts were irradiated to various doses of UVB radiations in a UV chamber fitted with 2 lamps emitting radiation in the range of 300nm and 320nm (312nm peak). Following UVB irradiation, the morphological changes and cellular proliferation/viability were monitored at different time periods post radiation exposure. Light microscopy revealed minor vacuolar changes at higher UVB doses. Cytoskeletal changes in the microfilament network were monitored by fluorescent microscopy with Phalloidin staining. Proliferation activity and viability of the 3T3 fibroblasts was measured quantitatively by spectrophotometry using Neutral Red Uptake (NRU) assay. In continuation, experiments have been planned to visualize the molecular details of microfilament and microtubule network reorganization post UVB irradiation using the Atomic Force Microscope (AFM).
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
