MORPHOLOGICAL BASIS OF AUTOIMMUNE MANIFESTATIONS IN VIRAL HEPATITISES

Abstract

The purpose of the study - to study morphological basis of extrahepatic lesions and autoimmune manifestations in chronic viral hepatitises Methods of performance. By indirect immunofluorescence ASMA, AMA, ANA, by enzyme immunoassay - the Anti-LKM-1 were determined. Cryoglobulins (CG) have been revealed with the use of qualitative visualization method, rheumatoid factor (RF) was determined in serum by semiquantitative latex agglutination on a slide. Patients underwent liver biopsy. A punction liver biopsy according to Mengini was performed with the subsequent histologic and morphological research of obtained liver bioptates with the use of an immunohistochemKal and immunocytochemical methods. Results. In patients with chronic viral hepatitis autoimmune manifestations (RF, CG, ASMA, ANA, AMA, anti-LKM-1) were identified at the same rate regardless of the degree of histological activity. In the liver bioptate in patients with chronic viral hepatitises the complex of regulatory T cells (CD4 + CD25 +), which indicates to the presence of morphological and functional basis for the formation of autoimmune manifestations against persistent viral infection was detected. Conclusion. Identified by ourselves autoimmune manifestations in the blood serum be embodied in the morphological changes of the liver tissue, at that the morphological changes characteristic of autoimmune hepatitises, are observed mainly in patients with chronic viral hepatitis B and C, with moderate and high histological activity.