Morphological and phylogenetic analyses reveal three new species of Diaporthe from Yunnan, China.

Shengting Huang,Xiuguo Zhang,Wenxiu Sun,Jiwen Xia

doi:10.3897/mycokeys.78.60878

Abstract

Species of Diaporthe have often been reported as plant pathogens, endophytes or saprobes, commonly isolated from a wide range of plant hosts. Sixteen strains isolated from species of ten host genera in Yunnan Province, China, represented three new species of Diaporthe, D. chrysalidocarpi, D. machili and D. pometiae as well as five known species D. arecae, D. hongkongensis, D. middletonii, D. osmanthi and D. pandanicola. Morphological comparisons with known species and DNA-based phylogenies based on the analysis of a multigene (ITS, TUB, TEF, CAL and HIS) dataset support the establishment of the new species. This study reveals that a high species diversity of Diaporthe with wide host ranges occur in tropical rainforest in Yunnan Province, China.

Highlights

The genus Diaporthe (Diaporthaceae Diaporthales) with asexual morphs previously known as Phomopsis spp. is based on the type species Diaporthe eres Nitschke (1870) from Ulmus sp. in Germany
A total of 3005 characters including gaps were obtained in the phylogenetic analysis, viz. intervening 5.8S nrRNA gene (ITS): 1–656, tubulin gene region (TUB): 657–1329, translation elongation factor 1-alpha (TEF): 1330–1860, CAL: 1861–2444, Table 1
Species diversity in Yunnan Province is high compared to other parts of China

Summary

Introduction

The genus Diaporthe (Diaporthaceae Diaporthales) with asexual morphs previously known as Phomopsis spp. is based on the type species Diaporthe eres Nitschke (1870) from Ulmus sp. in Germany. The genus Diaporthe (Diaporthaceae Diaporthales) with asexual morphs previously known as Phomopsis spp. is based on the type species Diaporthe eres Nitschke (1870) from Ulmus sp. The asexual morph is characterised by ostiolate pycnidia with cylindrical phialides often producing three types of hyaline, aseptate conidia called α-conidia, β-conidia and γ-conidia (Udayanga et al 2011; Gomes et al 2013). Diaporthe species have been reported as destructive plant pathogens, harmless endophytes or saprobes (Murali et al 2006; Udayanga et al 2012; Gomes et al 2013; Ménard et al 2014; Guarnaccia et al 2016; Torres et al 2016; Senanayake et al 2018). The biology and lifestyle of some of these fungi remain unclear (Vilka and Volkova 2015)

Objectives

Methods

Results

Conclusion