Morphological and osteological studies on the effect of acrylamide (ACR) on paternally treated fetuses of albino rat

Abstract

The present study investigates the effects of Acrylamide (ACR) on the skeletal system of the prenatal fetuses of 20th day of gestation of rat. The used rat were arranged in five groups; Control group (C) was the group treated orally by distilled water; G1 was the group of male treated daily with (10 mg/kg) of ACR orally for 10 days and then they allowed to mate with control females.G2 was the group of female treated daily with (10 mg/kg) of ACR orally for 10 days and then they allowed to mate with control males. G3 was the group of male and female treated daily with (10 mg/kg orally) of ACR orally for 10 days and then they allowed to mate with each other.G4 was the group of Pregnant female treated daily with (10 mg/kg) of ACR orally at (1st -20th) day of Pregnancy. At the 20th day of gestation, the uteri were removed by cesarean sections. For each mother, the number of fetal swelling in each horn, living and dead fetuses and early of late resorptions were recorded. Early and late resorptions were discerned according to their size. For morphological studies, fetuses either living or dead were counted, weighed and measured then morphologically examined for any external malformation. Stereoscopic examination for the obtained 20th day of gestation. Fetuses showed severe skeletal alterations included incomplete ossification for some bones of skull, vertebrae, fore and hind limbs and significant reduction in the length of most long bones of both limbs. The alterations indicated that ACR treatment induced growth retardation confirmed by great reduction in body weight and body length. The results suggest that ACR has Teratogenic effects on maternally treated 20-day-old albino rat fetuses.