Morphological and Molecular Screening of Soybean Genotypes against Yellow Mosaic Virus Disease

Abstract

Background: The growth and productivity of soybean are adversely affected by an array of biotic factors. Viruses are one of them as they cause great loss to the yield of soybean in India. The present study was conducted with an objective to identify yellow mosaic virus (YMV) resistant genotypes among the selected set of 53 soybean genotypes. Methods: The field screening was performed to identify YMV resistant genotypes. The field data was compared with molecular data recorded on the basis of gene specific SSR molecular markers. Result: During field study, 11 genotypes were found to be highly resistant, 26 resistant, 6 moderately resistant, 4 moderately susceptible, 3 susceptible, while three genotypes namely: JS335, JS 97-52 and RVS 2001-4 were found to be highly susceptible. In molecular analysis three genotypes viz.,: JS 20-29, JS 20-69 and JS 20-98 were found to be resistant against YMV. Among the polymorphic SSR markers the highest genetic diversity (0.4785) was observed with Satt554 while lowest genetic diversity (0.037) was observed with Satt308. Similarly polymorphism information content (PIC) was highest (0.364) in Satt554 and lowest (0.0363) in Satt308 among all polymorphic markers used for screening against YMV. The resistant genotypes identified in this study may be used as donor of resistance gene against YMV to develop improved genotypes which would stand as barrier against spread of the disease to newer areas and thus it can boost production and productivity of soybean in the country.