Morphological and Molecular Identification of Trichophyton mentagrophytes Isolated from Dermatophytes Patients in Garmian Area

Abstract

Conventional and molecular diagnosis considered as a complementary approach for making a final decision about causative agent of all microbial population, the present study was conducted in Kalar General Hospital, Dermatology unit and research laboratory center in Garmian University. Out of thirty clinically collected specimens only five samples displayed positive dermatophytic characters against microscopic and macroscopic as well as biochemical tests, such appearances of isolated colonies as white and brown color for both surface and reverse respectively, as well as, the colonies showed with a cottony texture which changed to powdery-granular colonies after two weeks incubation. Microscopic examination appeared numerous single-celled, spherical shaped microconidia were seen as clustered on both sides of hyphae, furthermore, multiseptate cigar shaped macroconidia and spiral hyphae were seen during the formation of granular colonies, biochemically analysis showed positive for urease and hair perforation testes. Molecular identification carried out via conventional PCR protocol by using the primers set ITS1 and ITS4 which result in 700bp in agarose gel electrophoresis for all isolates. PCR-RFLP carried out by means of using BstN1 digestion enzyme revealed four separated pattern bands 250,180,150 and 120bp. Sequencing of the ITS region in one of the isolated species revealed that similarity about 86% with Trichophyton mentagrophytes ATCC11481